Transfection is most commonly performed to express a protein of interest in cultured cells (or an animal model) through the use of a plasmid vector or mRNA. Expression of the protein in eukaryotic cells allows the recombinant protein to be produced with proper folding and post-translational modifications required for its function.

Transfection is a modern and powerful method used to insert foreign nucleic acids into eukaryotic cells. The ability to modify host cells’ genetic content enables the broad application of this process in studying normal cellular processes, disease molecular mechanism and gene therapeutic effect.

May 10, 2024 · In this article, we discuss the fundamentals of transfection, provide an overview of some of the commonly employed methods and a sample protocol. Finally, we consider some of the key applications of transfection.

Available transfection technologies are broadly classified into three groups: chemical, physical, and biological. No one transfection method can be applied to all cell types and experiments. Therefore, the ideal transfection approach should be selected based on your cell type and experimental needs.

Jul 20, 2021 · Transfection is a widely used laboratory cell culture technique that introduces foreign nucleic acids into cells. It is a powerful analytical tool enabling study of gene functions and gene products in cells.

To simplify the transfection optimization process, we have created a transfection selection tool that provides recommended transfection reagent with optimized protocol for 120 cell lines.

Transfection is a procedure that introduces foreign nucleic acids into cells to produce genetically modified cells. Transfection is a powerful analytical tool for study of gene function and regulation and protein function.

Transfection is the process of introducing nucleic acids into eukaryotic cells by nonviral methods. Using various chemical or physical methods, this gene transfer technology enables the study of gene function and protein expression in a cellular environment.

Get an overview and comparison of different transfection methods for eukaryotic cell cultures. Learn about their advantages, disadvantages, and applications.

While considering a suitable transfection reagent, it is important to identify the cell type and culture conditions for the assay. Rare cell cultures, neurons and primary cells are usually harder to transfect and hence will require reagents that can facilitate transfection even in …