Enhancing flap survival is essential in flap surgery. Cell-free fat extract (Ceffe), a “natural cocktail” of bioactive compounds derived from adipocytes, has demonstrated the potential to improve flap ...

Interestingly, in vitro EGFRi induced cell cycle arrest in NSCLC cells grown in monolayer, while 3D spheroids preferentially die upon inhibitor treatment. We find differential YAP nuclear localization ...

BACKGROUND: The microbially produced amino acid–derived metabolite imidazole propionate (ImP) contributes to the pathogenesis of type 2 diabetes. However, the effects of ImP on endothelial cell (EC) ...

The cells were then incubated for an additional 4 hours in a cell culture incubator. Absorbance was subsequently measured using a microplate reader to determine cell viability. The results, presented ...

Human recombinant epidermal growth factor (EGF) promotes cell proliferation as well as skin remodeling and healing. However, it’s unclear if EGF is stable in in vitro cell culture medium treatment ...

Hydrogel encapsulation is a rational approach that facilitates three-dimensional inoculation, arrangement, and culture of living mammalian cells for biomedical applications. However, strategies to ...

The march marked the opening of a four-day gathering known as the Pan-Amazon Social Forum (FOSPA), a semi-annual incubator where activists and leaders from Indigenous, Afro-descendant and other ...

LSCs were inoculated in six-well plates with sterile 4D-CTH in the experimental group and no treatment in the control group, and incubated overnight at 37°C in a cell culture incubator. After the ...

Cell Culture and Reagents. B16-F10 murine melanoma cells were purchased from the American Type Culture Collection (ATCC) and were cultured in DMEM+GlutaMax + 10% fetal bovine serum + 100 U ml −1 ...