Toward this goal, we describe a solution phase split-luciferase assay for native protein detection, where we fused fragmented halves of firefly luciferase to separate receptor fragments or ...

All data in Figure are representative of three biological replicates. Interactions between NopT and LjNFR1 and LjNFR5 were further verified using a Split– luciferase (Split–LUC) assay (Fig. 2C).

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When the wood is split open in the dark, it emits a green glow ... A substance called luciferin reacts with an enzyme called luciferase. This reaction creates an unstable product that gives off energy ...

SIMPL2 is an update of SIMPL that involves the use of the split luciferase enzyme for detection of protein interactions through luminescence. In addition to improving identification of ...

SIMPL2 is an update of SIMPL that involves the use of the split luciferase enzyme for detection of protein interactions through luminescence. In addition to improving identification of ...

This updated system, called SIMPL2, demonstrates significant improvements in performance. The incorporation of split luciferase significantly improves the operability and quantifiability of the assay.

The interaction between ZmCPK39 and ZmKnox2 has been validated by split-luciferase complementation and bimolecular fluorescence complementation assays. Importantly, the Mutator-induced ZmKnox2 ...

Reference: “Cell-Based Assay to Determine Type 3 Secretion System Translocon Assembly in Pseudomonas aeruginosa Using Split Luciferase” by Hanling Guo, Emily J. Geddes, Timothy J. Opperman and ...