These fundamental findings offer a novel mechanism by which rubrerythrin tubules encapsulate encapsulin to prevent oxidative stress in Pyrococcus furiosus. However, there are a few reasonable concerns ...

This study presents an important finding on the role of GATA4 in aging and OA-associated cartilage pathology. The evidence supporting the conclusions is compelling, with rigorous in vitro and in vivo ...

(23,24) However, demonstrated here is an approach accessible to most laboratories to set up in-house for a relatively ... Cells were resuspended in lysis buffer containing cOmplete Protease Inhibitor ...

Following administration of anesthesia without any noticeable response from their toes, the mice were carefully positioned on an automatically regulated heating pad set at 37 ... complete protease ...

Isolated OMVs were lysed using a solution containing 4% SDS, 100 mM Tris–HCl (pH 9), and a 1× protease inhibitor cocktail set III. The cell lysates were heated at 95 °C for 5 min and then sonicated ...

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and 20 mM imidazole), freshly supplemented with cOmplete EDTA-free Protease Inhibitor Cocktail. Subsequently, the cells were lysed by sonication and the lysate was clarified by centrifugation at (25 ...

containing protease Inhibitor Cocktail Set III, EDTA-Free (Calbiochem). Lysates were clarified by centrifugation at 8,000 × g for 15 min. For NLGN1 immunoprecipitations, 500–1,000 μg of total protein ...

Radioimmunoprecipitation assay buffer contained protease and phosphatase inhibitors (Thermo Fisher Scientific, Halt Protease and Phosphatase Inhibitor Cocktail, 78441 ... cold acetone for 10 minutes.

Cells were arrested in G1/S phase by treatment with thymidine (Sigma) or in mitosis by treatment with DME (Eg5 inhibitor III, Enzo Life Sciences ... 1% Triton X-100 and protease inhibitor cocktail ...

containing Complete Protease Inhibitor cocktail (Roche) and phosphatase inhibitor cocktails II and III (Sigma). Samples were cleared by centrifugation at 15,000 × g for 15 min at 4 °C and supernatant ...