The following criteria for PCR primer design were used: size of all primers was 18–22 nucleotides, G & C nucleotide content no more than 55%, and melting temperature was in the range of 56–60°C.

Note, the pH is 9.2 (the optimal for long PCR) without adjustment ... serve as the no-template control reaction and add 2 μl of primer pair 1. 3| Add 5.5 μl of genomic DNA at 10 ng/μl to ...

IT white papers, webcasts, case studies, and much more - all free to registered TechRepublic members. Planes, trains, and automobiles. They can be stressful, but this guide rounds up advice from ...