Subsequently, 30 µg of cell lysates were loaded onto S-Trap micro columns (ProtiFi, C02-micro); trypsin and lysyl-endopeptidase (Lys-C) solution (1:10 w/w ... containing a complete protease inhibitor ...

ECM or cell lysates were homogenized in radioimmunoprecipitation assay buffer with protease inhibitor cocktail ... L triethylammonium bicarbonate and digested with 0.8 μg trypsin/Lys-C overnight at 37 ...

C. Quantification accuracy of dimethyl labeled peptides in ... of all protein groups identified per condition in the dimethyl five‐plex setup, using Lys‐N as protease. Protein group intensities are ...

Protein identification by tandem MS after protease digestion is a robust tool in proteomics ... Some proteins may require other proteases, such as Lys-C, Asp-N, chymotrypsin, etc. If this is the case, ...

C. Cells were lysed and proteins were digested to peptides with Lys‐C and trypsin. Then, succinylated peptides were isolated directly from protease‐digested protein extracts by immunoaffinity ...

Chymotrypsin was from Sigma and Lys-C protease was from BDH Chemicals (Sweden). Recombinant human enteropeptidase light chain was a gift from Dr. Marine Gasparian (Gasparian et al., 2003).

Residual iodoacetamide was quenched by further addition of DTT (5.9 μl, 6 mM). Lys-C protease (1 mg/ml, 5 µl) in 500 mM ammonium bicarbonate, pH 8.0, was added, and samples were incubated for 1 h at ...

This structural and molecular dynamics study has great implications for the further development of Furin protease inhibitors ... and the peptide-based drug [succinyl-Phe-2-Nal-(Arg)3-Lys]-Lys-4-Amba ...

Effectively, by supplementing trypsin with Lys-C, we create improved trypsin. Trypsin is the most popular protease used in mass spectrometry because of its high proteolytic activity and cleavage ...