Sections were cut 3 μm thick in the Leica CM1850 cryostat at −20°C. Slides were incubated for 2 min in 4% PFA and washed with PBS three times for 10 min. Sections were permeabilized in 0.03% ...

Flash-frozen tissues were cryo-sectioned to 12 μm by using a LEICA CM1850 cryostat and thaw-mounted onto glass slides for subsequent LA-ICP-MS imaging experiments. Tissue homogenates of the organs, ...

25608-930) and sliced into 20 μm sections using a CM1850 cyrostat (LEICA Biosystems) at −20 °C. Sections were immediately mounted onto SuperFrost microscope slides (Fisher Scientific, cat.# 12-550-15) ...

Leica and Zeiss are two of the most established and respected names in photography, despite being best-known for different products. Leica is famous for its cameras, while Zeiss has made its name ...

Tissue cross-sections of 10 μm thickness were collected every 100 μm using a Leica CM1850 cryostat. For 3- or 7-day injured muscle tissues, mice were anesthetized with isoflurane. The injury was ...

Embedded samples were cryosectioned at 16μm using a cryostat (Leica CM1850) and adhered to positively charged glass slides (Fisherbrand ColorFrost Plus). Cryosections were stored at −20°C in freezers ...

For histological analysis, midline sections were cut using a Leica CM1850 cryostat (Leica Biosystems Inc., Buffalo Grove, IL). Three sections from each tissue section were placed on the plus side of a ...

10–20 μm sagittal sections were prepared using a cryostat (Leica CM1850). Immunohistochemistry was conducted as described previously (Hoch et al, 2017). Fluorescent images were acquired using the ...

Tissue sections (12 μm) were cut from gamma-irradiated rabbit-lung-biopsy specimens using a Leica CM1850 cryostat (Walldorf, Germany) and thaw-mounted onto stainless steel slides for MALDI ...

Frontal sections were cut on a freezing microtome (Leica CM1850, Germany) at 40 μm thickness and counterstained with cresyl violet (Sigma, USA). They were dehydrated and covered for subsequent ...

Tissue sections (7–10 μm) were cut from prepared optimal cutting temperature blocks using a Leica CM1850 cryostat and transferred to Colorfrost Plus Microscope slides (Thermo Fisher Scientific) and ...