the principle of flow cytometry; components of a flow cytometer; basics of fluorescence and how to interpret a dye excitation/emission spectrum; how data is displayed; compensation principles and ...

Compensation refers to correcting a phenomenon called fluorescence spillover in flow ... Compensation in flow cytometry is poorly understood; nevertheless, compensation is critical for several ...

replaces compensation, which is required in conventional flow cytometry to account for fluorescence spillover between channels.” Although autofluorescence can be a significant problem in typical ...

Recent advances in fluorescence-activated cell sorting (FACS) technology offer new and exciting approaches for understanding, monitoring and combating immune-related diseases. However, these ...

One of the primary objectives for the application of flow cytometry in any testing environment should ... Another important step in panel design is to optimize the fluorescence compensation and ...

This type of control determines the cut-off point between background fluorescence ... Additionally, compensation controls should be employed in a flow cytometry experiment. FMO controls are ...

Imaging cytometry 4: This type of cytometry combines traditional flow with fluorescence microscopy ... experiments with multiple fluorophores may require compensation. 5 Overlapping spectra can lead ...

Imaging cytometry 4: This type of cytometry combines traditional flow with fluorescence microscopy ... experiments with multiple fluorophores may require compensation. 5 Overlapping spectra can lead ...