The rapid identification of respiratory virus outbreaks is needed to enable rational and effective public health interventions. We developed new quantitative approaches for simultaneous ...

Development and Validation of a Cell-Free DNA Fragmentomics–Based Model for Early Detection of Pancreatic Cancer The following represents disclosure information provided by authors of this manuscript.

Twist FlexPrep™ Ultra-High Throughput (UHT) Library Preparation Kit The Twist Flex Prep ™ UHT Kit workflow is purpose-built to streamline and massively scale up Twist’s established enzymatic ...

Libraries were prepared using mRNA purified using polyA bead selection (New England Biolabs), using Illumina TruSeq v2 library preparation kit (Illumina). Sequencing was performed using HiSeq 2500 ...

Chromatin Immunoprecipitation Chromatin immunoprecipitation (ChIP) assay was conducted according to the manufacturer’s instructions (SimpleChIP Enzymatic Chromatin IP Kit, Cell Signaling Technology, ...

The plasma cfDNA was extracted from 2–4 mL plasma using the Quick-cfDNA Serum & Plasma Kit (ZYMO) and then stored at −80 °C. The concentration and quality of cfDNA were quantified by Qubit fluorometer ...

Abstract Cell-free DNA (cfDNA) is a rich source of biomarkers for various (patho)physiological conditions. Recent developments have used Machine Learning on large cfDNA data sets to enhance the ...

The same instrument, library preparation kit, and data analysis pipeline were used at all 9 institutions for plasma cfDNA mNGS. Patients were excluded from the subject group if they died within 24 ...