Fluorescence in situ hybridization (FISH) is a method to localize nucleic acid targets in fixed cells for cytogenetic or gene expression studies. It relies on fluorophore-labelled DNA or RNA ...

Cryo-ET elucidates in situ assembly of MacAB-TolC efflux pump in E. coli, revealing antibiotic-induced formation of MacA-TolC subcomplexes and dynamic MacB binding, which provides structural ...