The following criteria for PCR primer design were used: size of all primers was 18–22 nucleotides, G & C nucleotide content no more than 55%, and melting temperature was in the range of 56–60°C.

The ELN includes native tools for CRISPR guide design, plasmid construction, compound modeling, genome browsing, and PCR primer design—making it an ideal solution for molecular biology, genetic ...

The global Oligonucleotide Synthesis Market is valued at USD 7.78 Billion in 2024 and is projected to reach a value of USD 21 ...

Primer design and information The Modified Tetra-Primer Assay (MTPA) PCR primer design tool was used to design primers (Tanha et al., 2015) using GenBank sequence of ACE2 rs2285666 and ACE2 rs2106809 ...

An AI-based EGFR biomarker tool could preclude the need for a rapid PCR test in nearly half of advanced lung adenocarcinoma cases, a study has suggested.

An ASCO provisional clinical opinion offers timely clinical direction to ASCO's membership following publication or presentation of potentially practice-changing data from major studies. This ...

Antibody–siRNA conjugates (ARCs) are a type of promising drug modalities for cancer therapy. However, initial reports of ARCs present the gene knockdown effect only in limited tissues (e.g., muscles) ...

This important study looks into the effect of exogenous CoA on the response of TLR4-activated macrophages. Specifically, CoA enhances the LPS response by examining metabolomics, 13C tracing, and ...

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Abstract Alginate lyase is a critical enzyme that catalyzes alginate degradation; however, its low thermal stability limits its industrial application. This study proposes a feasible and efficient ...