2 Growing iPSCs in vitro requires a culture medium, growth factors, and an extracellular matrix. The culture medium quality profoundly impacts cell growth, viability, and differentiated cell type.

Photostability of mitochondrially localized EGFP had a low dependence on medium composition and was higher than that of cytosolic EGFP in DMEM (Table 1 and Supplementary Fig. 1). We observed an ...

Centrifuge the cells at 200g for 5 min at 15–25 °C and resuspend the pellet in 10 ml MEF medium supplemented with 4 ng/ml bFGF. To prepare the appropriate culture vessels for plating the ...