In all cases, a protein purity level of >85% was obtained from one-step purification. Low endotoxin levels of 0.01 EU/µg were achieved without additional endotoxin removal steps (Figure 2). Figure 3.

These tests enable the detection of endotoxins, the pyrogenic components found in the outer membrane of Gram-negative bacteria. In 1977, the FDA approved the use of the limulus amebocyte lysate ...

Charles River eventually published the study that Klug said he encountered, which compared different assays’ detection endotoxins in unpurified manufacturing facility water and concluded that ...

It streamlines endotoxin testing in a platform that is very easy to set up, use and maintain. Microfluidic automation delivers high throughput, rapid assay setup, reduced hands-on time and simple ...

Achieving endotoxin automation and analysis without sacrificing compliance is no easy task. However, the Veolia Siever Eclipse bacterial endotoxins testing platform combines state-of-the-art ...

Exotoxin A is an extracellular enzyme that is produced by most clinical strains of Pseudomonas aeruginosa. It is a single-chain polypeptide (molecular weight, 71,000) with A and B fragments that ...

2. Kass, E. H. Effect of corticosteroids and of hormones of pregnancy on lethal action of bacterial endotoxin. Ann. New York Acad. Sc. 88:107–115, 1960.

M. tuberculosis, which kills more than 1 million people a year, uses the ESX-4 type VII secretion system to transports its potent exotoxin. Six years ago, Michael Niederweis, Ph.D., described the ...

In this review of the mechanisms underlying pyrogenic tolerance to bacterial endotoxins, two earlier concepts require modification. (1) Granulocytes are the major source of endogenous pyrogen release ...

In this episode of C&EN Uncovered, host Craig Bettenhausen speaks with C&EN assistant editor Laurel Oldach about the use of horseshoe crab blood in pharmaceutical endotoxin testing, the challenges ...