To generate a tissue specific knockout mouse, researchers breed a mouse bearing a Cre transgene under a tissue- or cell-type specific or inducible promoter (A) with a homozygous floxed mouse (B). The ...

The Cre/lox system is widely used in mice to achieve cell-type-specific gene expression. However, a strong and universally responding system to express genes under Cre control is still lacking. We ...

A new editorial paper was published in Oncoscience (Volume 10) on November 14, 2023, entitled, "Be mindful of potential pitfalls when using the Cre-LoxP system in cancer research." In this new ...

To discover the function of a gene researchers turn it off and observe the consequences. Often genes have multiple functions that differ depending on a tissue and age. Some genes are essential to ...

Cre-loxP, a powerful tool for preclinical cancer research, possesses intrinsic pitfalls that can alter result accuracy, according to an editorial paper in Oncoscience. Cancer and other diseases could ...

Before CRISPR-based methods took center stage in the genome editing field, other bio-inspired tools such as zinc finger nucleases and Cre-lox system recombinases made genomic engineering possible.

We have created a stable transgenic rag2-EGFP-mMyc zebrafish line that develops GFP-labeled T cell acute lymphoblastic leukemia (T-ALL), allowing visualization of the onset and spread of this disease.

“One limitation [of Cre-LoxP], the focus of this editorial, is the potential loss of fidelity of Cre recombinase expression especially in the context of modeling cancer in mice.” “It is based on ...