Inoue H et al. (2000) A novel method of virtual histology utilizing laser-scanning confocal microscopy for untreated fresh specimens of the gastrointestinal mucosa. Endoscopy 32: 439–443.

Confocal laser scanning microscopy (CLSM) is the most widely used confocal technique (Figure 1b). A single point of laser light is used to illuminate a sample. In this technique, ...

For this to be a ‘true’ laser scanning microscopy, the spot needs to be scanned over the FOV.. this is usually accomplished with a pair of galvo mirrors in the illumination path and an image ...

The basic scheme here is known as confocal laser scanning fluorescence microscopy, where a laser at one wavelength excites fluorescent tags bound to structures in a sample.

Unlike the light microscopes that use visible light to illuminate the sample, LSCM instead utilizes a focused scanning laser to illuminate a subsurface of a three-dimensional (3D) specimen. The visual ...

Laser confocal scanning microscopy is a reliable, noncontact technology to observe and measure fine surface topographies. With all the evolutionary and revolutionary progress going on in various ...

More information: Wei Ren et al, Expanding super-resolution imaging versatility in organisms with multi-confocal image scanning microscopy, National Science Review (2024). DOI: 10.1093/nsr/nwae303 ...

A new s²ISM imaging method enhances laser scanning microscopy with superior resolution and optical sectioning, offering ...

However, the availability and diversity of laser types accommodated by laser scanning confocal microscopy mean that it remains the most popular option. Image Credit: souvikonline200521 ...

Current concepts in light and electron microscopy and scanning probe techniques. Theory and practice of fluorescence (including confocal and multi-photon), atomic force, scanning and transmission ...