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High-throughput single-cell DNA methylation profiling
Scale Bio offers a user-friendly and fully kitted solution that makes single-cell methylation analysis ... The Agilent Tapestation DS5000 High Sensitivity DNA Kit was used to determine fragment ...
ahajournals.org1y
Single-Cell Transcriptome Reveals Potential Mechanisms for Coronary Artery Lesions in Kawasaki Disease
In this study, we present the first single-cell transcriptomic analysis of PBMCs from patients ... DNA assay (Thermo Fisher Scientific) and the high-sensitivity DNA kit of Bioanalyzer 2200 (Agilent).
The EMBO Journal1y
IL-27 produced during acute malaria infection regulates Plasmodium -specific memory CD4
Single‐cell RNA‐seq libraries were prepared using the Chromium Next GEM Single Cell 5′ Reagent Kits v2 (Dual Index; 10X Genomics), SPRI reagent (Beckman Coulter Genomics), and High Sensitivity DNA ...
ahajournals.org1y
Age‐Associated Changes in Endothelial Transcriptome and Epigenetic Landscapes Correlate With Elevated Risk of Cerebral Microbleeds
The RRBS library was analyzed with Bioanalyzer High‐Sensitivity DNA Analysis kit (Agilent), and the concentration was determined using the Qubit HS DNA kit (Life Technologies). The sequencing was ...
Scientific Research Publishing1y
Diversity Analysis of Endophytes in Wheat Infected by Powdery Mildew ()
Therefore, to fully understand the bacterial community, recent years culture-independent molecular approaches based on 16S rDNA analysis ... Kit (OMEGA Bio-Tek, USA, www.omegabiotek.com) and analyzed ...
The New York Times2y
DNA evidence is a no-go zone in the trial.
“It is possible that the results of further DNA analysis using Mr. Trump’s DNA sample would be entirely inconclusive,” he wrote. The upshot is that DNA evidence cannot be brought up by ...
eLife3y
Regulatory T-cells inhibit microglia-induced pain hypersensitivity in female mice
Behavioral analysis was done 2 hr after injections ... Quality was determined with the Agilent High Sensitivity DNA Kit on a Bioanalyzer (Agilent) and concentrations were measured on Qubit (Thermo ...
Frontiers3y
Comparative Genomic Analysis Uncovered Evolution of Pathogenicity Factors, Horizontal Gene Transfer Events, and Heavy Metal Resistance Traits in Citrus Canker Bacterium ...
Purity and quantity of DNA samples were estimated using the Qubit dsDNA HS Assay Kit (Thermo-Fisher Scientific) and Agilent BioAnalyzer 2100 High Sensitivity DNA Kit ... with the core-genome ...
Frontiers4y
Assessing the Impact of Data Preprocessing on Analyzing Next Generation Sequencing Data
A Quality control Agilent 2100 Bioanalyzer with a High Sensitivity DNA Kit was used for quality control (Agilent Technologies, Santa Clara, CA, United States). NGS analysis was performed on a ...
The Lancet5y
Celastrol-induced degradation of FANCD2 sensitizes pediatric high-grade gliomas to the DNA-crosslinking agent carboplatin
In addition, our results suggest that celastrol treatment stalls ongoing replication forks, causing sensitivity to DNA-crosslinking in ... RNA-quality was analyzed with the Agilent 2100 Bioanalyzer ...
ascopubs.org6y
Detection of Circulating Tumor DNA in Patients With Leiomyosarcoma With Progressive Disease
Extracted DNA was quantified using Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific, Waltham, MA). High-molecular-weight DNA contamination of cell-free DNA was determined by Bioanalyzer ...