A capillary western blot (Wes) technology has recently been validated for analyses of cell culture lysate proteins, but whether it is reliable for human tissue proteins is unknown. We compared ...

Since its introduction in 1979, the traditional Western blot has remained a lengthy and laborious analytical process. But by taking an automated approach, researchers can reduce errors, enhance data ...

The southwestern blot is so named as it encompasses elements of both the Southern and western blots. The technique involves the identification and characterization of DNA-binding proteins using DNA ...

Western blot analyses on cytoplasmic and nuclear fractions revealed that Akt activity (represented by phospho-Akt S473) is higher in the cytoplasm than in the nucleus.

Western blot and its use in cancer research and pain management are reviewed in this article, ... Li J, Cheng D, Zhu M et al. OTUB2 stabilizes U2AF2 to promote the Warburg effect and tumorigenesis via ...

Although speed is not of utmost importance in chemiluminescent Western blot imaging (as it usually demands extended exposure times), it can still be fundamental when dosing high-throughput ...

Western blot (WB) analysis was used to detect the expression of related proteins, enabling a preliminary exploration of Ejiao’s effects and regulatory mechanisms.

I joined the University of North Carolina at Chapel Hill in 2021 as a neuroscience graduate student. That fall, I rotated in Graham Diering’s lab, focusing on the role of sleep on normal development ...

Reliable assessment of changes in target protein levels by western blot requires measurement of both the target and loading control proteins in the linear dynamic range. Stain-free technology is a ...

AUSTIN, Texas, Sept. 3, 2014 /PRNewswire/ -- Western Blot Service CRO, a biology contract research organization based in Austin, Texas, announced today that it is consolidating its years of expert ...

A new, easier method uses Stain-Free technology for total protein Western blot normalization. Benefits of Stain-Free Technology Stain-Free technology is a unique in-gel chemistry that is available ...

Once your Western blots produce a single band, you should confirm whether that band is your protein. Jensen says the best control is an siRNA knockdown or loading a knockout sample. Problem #1 ...