Insulin treatment leads to phosphorylation of AKT, a known signaling factor activated by insulin. Cells were treated with a serial titration of insulin for 10 min before they were harvested and ...

Chronic obstructive pulmonary disease (COPD) is an irreversible inflammatory lung disease. Studies have shown that ...

WESTERN Ky. (WFIE) - Kentucky Transportation Officials say some roads are starting to reopen as rain moves out of the region. However, they say further closures should be expected, especially in ...

Background— Celecoxib has been shown to have antitumor effects that may be mediated through the cyclooxygenase-independent inhibition of Akt signaling. Here, we examined the effects of celecoxib on n ...

Immunohistochemistry was used to analyze the expression of ACO3 and AKT. Western blot was used to analyze the expression of ANGPT1, ANGPT4 and KDR; reverse transcription polymerase chain reaction ...

Finally, phosphorylation of Nrf2 and CREB, mitochondrial apoptosis-related markers and JNK/AKT/ERK pathway activation were examined by qRT-PCR and Western blot. As a result, flow cytometry and TUNEL ...

The related protein levels of the cell cycle, apoptosis, autophagy and AKT/GSK3β pathway were determined by Western blot. The anti-tumor activity of Cos was evaluated by subcutaneously ...

After that, Western blot was used to detect ERM, changes in the concentration of PI3K and Akt proteins. Results: Compared with the normal group, the expression levels of ERM, PI3K, and Akt proteins in ...

2.4. The Expression of HIF-1α, PI3K, Akt, p-PI3K, p-Akt and Other Proteins Were Detected by Western Blot ACHN cells were inoculated into culture flask at a density of 1 × 10 6 per flask, and the four ...

we introduced a PI3K/Akt activator (740Y-P) in mice and adopted RT-qPCR, Western blot analysis, and IHC, which revealed that the protein expression levels of VEGFA, PI3K p-Akt, and Akt were all ...