A number of variables impact cell and tissue culture, from reagent and cell-line selection to experimental design and validation strategies. 2 One that frequently goes overlooked is the incubator.

The responsibility for procurement of tissue culture supplies lies with each individual investigator. Investigators may also be assigned their own incubator depending on availability, or they may ...

Image: Anne MacNamara/R. Ian Freshney Like most researchers, you probably hate tissue culture work. But, if such 'trivialities' as experimental quality and reproducibility are important to you, then ...

The Tissue Culture Core Laboratory maintains active cell cultures of many types of mammalian cell lines as well as frozen stocks of a larger cell library deposited by BCM and Molecular and Cellular ...

1| Harvest exponentially growing cells 24 h before transfection by trypsinization and replate them at a density of 1 × 10 5 to 4 × 10 5 cells/cm 2 in 60-mm tissue culture dishes or in 12-well ...