SIMPL2 is an update of SIMPL that involves the use of the split luciferase enzyme for detection of protein interactions through luminescence. In addition to improving identification of ...

He extracted two components from beetle abdomens, naming the substrate luciferine and the enzyme responsible for the reaction luciferase. 2 Not all bioluminescent marine organism systems can be used ...

It is based on a split-luciferase system, whereby the light-producing enzyme is split in two and attached to the interacting proteins of interest, so that when the interaction occurs, the two ...