A deeper look into the apparent self-defense mechanisms of these bacteriophage-resistant bacteria revealed their secret weapon: a group of enzymes called restriction endonucleases, or restriction ...

Historical examples include DNA and protein sequencing, restriction enzymes, PCR and the two-hybrid assay. The most recent addition to the methods hall of fame is RNA interference (RNAi).

but they did not identify and isolate restriction enzymes until the late 1960s. 3 In 1970, Hamilton Smith and Kent Wilcox from Johns Hopkins University discovered “endonuclease R” (later named HindII) ...