PCR was developed in 1983 ... the Promega website will have a Plexor Primer Design tool that will recommend the primer sets, including fluorophores, to be ordered for one or several target ...

The findings, published on 15 Feb 2025 in Frontiers of Computer Science, address a critical challenge in quantitative PCR ...

The findings, published on 15 Feb 2025 in Frontiers of Computer Science, address a critical challenge in quantitative PCR (qPCR) primer design. Unlike existing software that requires manual ...

High coverage, easy assay design and assay compatibility provide a high degree of flexibility; at the same time, in silico PCR ensures high specificity of primer and probe combinations in the ...

The recommended final concentration is 1-10 percent. Formamide weakens base pairing and increases primer annealing specificity, thus enhancing PCR amplification for templates with high GC content.