To carry out real-time PCR, researchers have to choose not only what primers to design (see Box 2) but also what detection chemistry to use. In many cases these decisions will be influenced ...

High coverage, easy assay design and assay compatibility provide a high degree of flexibility; at the same time, in silico PCR ensures high specificity of primer and probe combinations in the ...

LGCs consultative approach to working with you, together with an industry-leading portfolio of probes and oligo modifications gives you confidence that you are getting the best PCR assay design to ...

Extension: Nucleotides are added to the primers, which serve as extension points to continue new strand synthesis. Endpoint analysis: Scientists analyze PCR products typically using agarose gel ...

“But the idea that you could amplify a specific targeted DNA sequence using an oligonucleotide primer and DNA polymerase was not at all obvious.” Erlich led the team of scientists at the Cetus ...