To carry out real-time PCR, researchers have to choose not only what primers to design (see Box 2) but also what detection chemistry to use. In many cases these decisions will be influenced ...

PCR-Machines are based on Polymerase Chain Reaction (PCR) and produce copies of genes exponentially. The PCR cycling reactions are carried out in an automated thermocycler, which is capable of ...

Figure 1: Amplification of complex gene libraries by conventional PCR and emulsion PCR ... ice-cold components for the alternative protocol (Box 1). Pipet the emulsion into the wells of a PCR ...