The cells were lifted in 500 μl phosphate-buffered saline (PBS) by gentle pipetting. Cell suspensions were kept on ice until analysis by flow cytometry (which was all completed within 1 h after ...

Flow cytometry enables researchers to label proteins of interest using fluorophore-conjugated antibodies and other fluorochromes. Much like immunohistochemistry, which produces two- or three ...

Multiplexing with these unstable fluorophores can impact conventional and spectral cytometry data quality ... for 1 hr in PBS + 1% BSA, Bio-Rad Stain buffer (#BUF073) or Brilliant stain buffer ...

This small device could make flow cytometry more accessible, affordable and faster, providing intriguing possibilities for researchers working in remote locations, field researchers and for ...

Flow cytometry is an analytical method that allows cells to be quickly analyzed and categorized by lasers as they pass in single-file. The way in which the laser light is scattered can be used to ...

Flow cytometry effectively detects CTCs in melanoma patients, offering a rapid, cost-effective alternative to molecular biology. CTCs are present across all melanoma stages, with a significant ...

Learn about our Editorial Policies. In addition to cell analysis, cell sorting, sometimes referred to as fluorescence-activated cell sorting (FACS), is another common application of flow cytometry. 2 ...