This is a preview. Log in through your library . Abstract The use of new powerful nested polymerase chain reaction (PCR) techniques to identify and screen for prevalence of parasites has a huge ...

Nested PCR protocol and increasing the number of cycles may also help. Dr. Mahajan: Every now and again we receive samples for testing that are either in an uncharacterized matrix or in a matrix known ...

Sandal (Santalum album L.) is a valuable tree of southern India. It is severely affected by sandal spike (SAS) disease, which is characterized by witches' broom symptoms. SAS is caused by a ...

When creating a PCR protocol, users normally concentrate on the template DNA, reaction mix (buffer, dNTPs, taq polymerase), primers, probes, and the thermocycler, which is the main instrument used to ...

The polymerase chain reaction -- A brief comparison between in vivo DNA replication and in vitro PCR amplification -- The PCR in practice -- The different types and varieties of nucleic acid target ...