First developed more than four decades ago as a biological research technique to amplify nucleic acids, polymerase chain reaction (PCR) technology still has untapped potential, with many exciting ...
As in baking, temperature is key to PCR: high temperatures cause denaturing; cooler temperatures favour annealing; and most polymerases will only operate within a narrow temperature window ...
Today, PCR is ubiquitous in molecular biology laboratories ... DNA polymerase from Thermus aquaticus—the now-famous Taq polymerase—which survives denaturing temperatures and copies DNA at 70°C. 2 “We ...
Denaturing gradient gel electrophoresis (DGGE) is a molecular fingerprinting method that separates polymerase chain reaction generated DNA products. The polymerase chain reaction (PCR) of ...