Nick recognition by vaccinia DNA ligase and Chlorella virus DNA ligase also depends on occupancy of the AMP binding pocket on the enzyme — i.e., mutations of the ligase active site that abolish the ...

Francisco J. López de Saro, Mike O'Donnell, Interaction of the β Sliding Clamp with MutS, Ligase, and DNA Polymerase I, Proceedings of the National Academy of Sciences of the United States of America, ...

The transcription enzyme RNA polymerase stalls at DNA lesions, hindering their repair. Accessory factors dislodge the enzyme by pushing it forwards, but a study finds that pulling it backwards may ...

When TV shows like CSI: Crime Scene Investigation portray DNA processing in the forensic lab, the results appear instantly with a DNA match wrapping up the case in hours. However, the ...

These are probably V- or U-shaped and form as the polymerase copies a section of one chain and then travels back along a second before moving on. After cleavage, half of each new segment pairs with ...

DNA 'scrunching' occurs as RNA polymerase selects a position to begin synthesizing RNA Massive data analysis reveals basic processes in biology Date: March 3, 2016 Source: Children's Hospital of ...

Now, researchers present a high-resolution structure of the monkeypox virus DNA polymerase holoenzyme—a complex that plays a key role in the genome replication process of the virus.

PCR, or Polymerase Chain Reaction, is a revolutionary molecular biology technique that allows for the rapid amplification of specific DNA sequences. It is an essential tool in various fields, ...

Journal reference: Rosenstein, A.H., et al. (2021) Fidelity of a bacterial DNA polymerase in microgravity, a model for human health in space.