The genetic connections between DNA repair pathways and human cancer predisposition have fueled interest in the proteins that recognize and repair specific sites of DNA damage. The repair enzymes are ...

This 'recombinant DNA technology' was initiated soon after the discovery of DNA ligase 1 and restriction ... III and antibody-bound Taq DNA polymerase, which allow for one-step thermocycled ...

So far, we've discussed using DNA polymerase for sequencing reactions ... The idea is that a specific enzyme, T4 DNA ligase, will link two neighboring pieces of DNA, but only if they're base ...

new DNA is made by the DNA polymerase making short 5’-3’ fragments, called Okazaki fragments. Then a different enzyme, DNA ligase, connects the Okazaki fragments together to form the new 3 ...

coli DNA polymerase I and the gaps are closed by T4 DNA ligase. Such methods where two concerted enzymes are used can only be employed in the presence of a 3’ OH or 5’ P terminus that has not ...

Forensic DNA profiling uses polymerase chain reaction (PCR) to amplify short tandem repeats (STRs), which are highly variable DNA regions, typically with four repeating bases. This variability helps ...

In the latest shake-up for scientific dogma, research has demonstrated that eukaryotic cells can convert RNA sequences back into DNA, through a misunderstood, ‘error-prone’ polymerase. Historically, ...

Now his team has built a mirror version of the 775-amino acid Pfu DNA polymerase, which Zhu says is the largest functional protein to be chemically synthesized to date. In its natural form ...

More information: Chuyao Fan et al, Bioorthogonal information storage in l-DNA with a high-fidelity mirror-image Pfu DNA polymerase, Nature Biotechnology (2021). DOI: 10.1038/s41587-021-00969-6 ...

Investigators have detected donor DNA after transfusion with a process called polymerase chain reaction (PCR) that amplifies minuscule amounts of genetic material for detection and identification ...