The key part of a confocal microscope is the pinhole placed in front of the detector at the confocal plane of the objective. Thanks to this pinhole, only light emitted from the small focal spot is ...

Our basic Confocal Laser Scanning System includes an electronics control unit, an expandable dual PMT module, a 16-position pinhole wheel, a two-channel (488 nm & 642 nm) laser source, and the ...

While confocal microscopy uses a pinhole to reject out-of-focus light to generate the optical section, a multi-photon (or 2-photon) microscope uses a pulsed infrared laser to stimulate fluorescence ...

A high-performance spinning disk confocal in an enclosed ... There are dual Nipkow disks with 50 or 25-micrometer lens pinhole arrays. The stage is motorized in X, Y, Z and coupled with Olympus’ ...

This 3D-printed confocal laser scanning microscope is a ... and a photodiode detector behind a pinhole. The detector feeds a homebrew trans-impedance amplifier that captures data at millions ...

The LSM 9 family with Airyscan 2 from ZEISS provides more options to enable the perfect balance of speed and resolution for today’s confocal-imaging needs. By combining the pinhole-plane imaging ...

By collecting the information of a pinhole-plane image in addition to a priori knowledge of the detection point spread function, the Airyscan detector increases both the spatial resolution and the ...

The Airyscan PrincipleA classic confocal microscope illuminates one spot on the sample to detect the emitted fluorescence signal. Out-of-focus emission light is rejected at a pinhole, the size of ...