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White light interferometry (WLI) and confocal microscopy or laser scanning confocal microscopy (LSCM) are the other two key techniques providing a 3D surface representation from a microscope image.
The central idea of a confocal microscope is that the light is focused to increase the signal intensity from a sample. In many other types of microscopy, such as fluorescence microscopy ...
The Department of Biology at Saint Louis University offers a Leica SP8 confocal microscope with resonant scanner for academic and commercial use. Live-cell imaging can be performed using a stage ...
Cross section analysis of commercial plastic, showing the capability of confocal Raman microscopy for analysis of µm thick layers However, this setup doesn’t allow for control over the volume sampled.
Images up to 4096 x 4096 pixels. Training is meant for people who have a solid understanding of confocal microscopy, including the preparation of the samples and dye selection. The purpose of training ...
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Their findings are published in Nature Communications. In life sciences, confocal fluorescence microscopy (CFM) is widely ...
Confocal and widefield microscopy each offer unique benefits and many researchers often require access to both technologies. In this issue of Under the Microscope, Dr Maria Giubettini, Application ...
In confocal Raman microscopy, a Raman spectrometer is coupled to a standard optical microscope, which leads to a high magnification of the sample and subsequent Raman analysis. Combining Raman ...
BrisSynBio has access to the confocal and light microscopy service provided by the Wolfson Bioimaging Facility in the Biomedical Sciences Building, and has helped fund the expansion of this centre and ...
The basic scheme here is known as confocal laser scanning fluorescence microscopy, where a laser at one wavelength excites fluorescent tags bound to structures in a sample. Light emitted by the ...
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