FOR the paper chromatography of steroids several colour reagents are used, such as 2,4-dinitrophenyl-hydrazine, Zimmermann's reagent, phosphomolybdic acid, antimony trichloride, etc. Their common ...

A pencil line is drawn, and spots of ink or plant dye are placed on it. There is a container of solvent, such as water or ethanol. A pure substance will only produce one spot on the chromatogram ...

A paper chromatogram can also be used to identify substances by comparing them with known substances. Two substances are likely to be the same if: they produce the same number of spots ...