Western blot sample preparation: Protein extraction and quantification Sample preparation is a crucial first step in performing an accurate western blot. 4 After extracting total protein from the ...

Chemiluminescent western blot detection concerns light emission caused by a chemical reaction (HRP and luminol). This light is frequently faint and demands sensitive imaging to capture the bands ...

Mariella is an assistant editor at The Scientist. She has a background in neuroscience, and her work has appeared in Drug Discovery News and Massive Science. View full profile. Learn about our ...