3b). Figure 3: Airy disk/aberration and power transmission measurements. Research-grade inverted biological/life science optical microscope (Nikon, TE2000E) with the following components ...

The microscope setup requires 2 h (1 h for laser ... we selected the PSF method. The size of the Airy disk and the spacing between the diffraction rings will depend on three things: (i) the ...

The Airyscan Principle A classic confocal microscope illuminates one spot on the sample to detect the emitted fluorescence signal. Out-of-focus emission light is rejected at a pinhole, the size of ...

The inverted Nikon spinning disk confocal microscope is equipped with four laser lines (405, 488, 561, and 647 nm), 4x-40x air objective lenses, and 60x NA1.40 and 100x NA1.45 oil objective lenses.